Prepare cell culture as necessary for experimental protocol
Prepare cell culture plate template, including reference blanks, in LiCellMo software
A well with media only/no cells. Must have defined amounts of glucose and lactate. Note: reference blanks are required.
If using multiple media types, include at least one blank well for every type of media being used.
Media with defined glucose and lactate concentrations. Calibration A with glucose and lactate at set concentrations, and Calibration B with glucose and lactate at different set concentrations (must be different than the concentrations prepared for Calibration A). No cells used for Calibration solution prep.
Note: Preparation of Calibration solutions must fall within glucose and lactate LOD.
Glucose LOD: 1-27mM
Lactate LOD: 1-15mM
Each calibration runs 24 hours for a total of 48 hours.
Only in the event of multiple errors across several runs, or at the suggestion of a PHCNA certified technician.
1 mL is recommended, but as little as 0.5 mL can be used